Ultra-high parameter, single cell multiomic phenotyping

Characterize complex cell suspensions by profiling hundreds of cell surface proteins along with gene expression, at single cell resolution. Gain a high-resolution view of complex biology, cell by cell, and broaden your discovery.

Workflow

Prepare your sample

Label your cells with TotalSeq™–B/C antibodies conjugated with a unique oligonucleotide barcode. You can also enrich your populations of interest by staining with antibodies for flow cytometry at the same time. Once the cells are washed, you can proceed to 10x library construction.

Construct your 10x library

Construct 10x barcoded libraries using our reagent kits and a compatible Chromium instrument. Each member of the Chromium instrument family encapsulates each cell with a 10x barcoded Gel Bead in a single partition. Within each nanoliter-scale partition, cellular transcripts undergo reverse transcription to generate cDNA for both mRNA and cell surface proteins, each of which shares a 10x Barcode with all cDNA from its cell of origin.

Sequence

The resulting 10x barcoded libraries are compatible with standard NGS short-read sequencing on Illumina sequencers for profiling of thousands of individual cells.

Analyze your data

Convert raw sequencing data to biologically meaningful insights with Cell Ranger. Cell Ranger processes data from both gene expression and cell surface protein libraries.

Visualize your data

Use our Loupe Browser to define and compare cell types based on gene expression and cell surface proteins.