We recently discussed the key features of our Visium Spatial Gene Expression and Xenium In Situ spatial transcriptomics...
Single-cell research is opening up a revolutionary new avenue for understanding gene expression at the individual cell l...
Philosophical, theological and scientific thoughts about the beginning of life have captivated societies for millenia. It is generally agreed upon that the earth is 4.5 billion years old, and had conditions conducive to life by about 4.3 billion years ago.
Chromium Single Cell Immune profiling allows simultaneous analysis of T-cell receptor (TCR) sequences and gene expression profiles from the same individual cells, enabling researchers to measure clonal expansion and identify often overlooked immune cells in the tumor microenvironment.
10x Genomics has made a scientific breakthrough to resolve limitations of former methods when they introduced BEAM (Barcode Enabled Antigen Mapping).
Spatially transcriptomics is crucial for understanding how cell location influences gene expression, development, and disease. This field has evolved from early techniques like radioactive in situ hybridization to advanced methods such as sequencing-based Visium Spatial and imaging-based Xenium In Situ. These technologies allow researchers to analyze gene expression within the natural cellular context, revealing new biological insights across various fields, including developmental biology, cancer, and neuroscience. Choosing the right method depends on research goals, with sequencing-based methods suited for hypothesis generation and imaging-based methods for detailed hypothesis testing and specific target analysis.
Spatial transcriptomics, a revolutionary technique that unveils gene activity within tissues, is transforming biological research. 10x Genomics offers two powerful solutions, Visium HD and Xenium In Situ, that provide a more holistic view of gene expression. Visium HD analyzes the entire transcriptome across large areas, making it ideal for initial discovery. Xenium In Situ, on the other hand, focuses on a targeted set of genes with exceptional resolution, perfect for validating prior findings. By using these complementary methods together, researchers can unlock a deeper understanding of gene expression within tissues.
Understanding tumor invasiveness is challenging due to subtle heterogeneity. The Xenium In Situ platform from 10x Genomics integrates single-cell resolution with spatial gene expression to uncover detailed heterogeneity in tumors. In a breast cancer study, it identified a rare triple-positive region (HER2+/ER+/PR+), crucial for prognosis and treatment. Xenium uses high-sensitivity padlock probes to create a spatial map of transcripts at subcellular resolution and preserves tissue for further analysis. This powerful tool enhances cancer research by providing comprehensive molecular insights and improving diagnostic and treatment strategies.
With Visium's high-resolution spatial capture technology and intuitive software, researchers can visualize gene and protein expression like never before. From understanding cellular dynamics to deciphering disease mechanisms, Visium empowers researchers across diverse fields to embark on a journey of discovery, uncovering the secrets of tissue biology and disease pathology with style and precision.
Those limitations are why our team developed this single cell RNA sequencing (scRNA-seq) protocol for PFA-fixed tissues. In this blog you’ll get a quick snapshot of how it offers you access to more samples, lets you process them on your schedule, retains your sample quality throughout storage and transport, and provides the greatest sensitivity of any commercial fixed RNA profiling protocol. Or, if you’re ready to jump right in, we have a direct link to the protocol and a webinar that goes into greater depth (and also offers a preview of FFPE tissue compatibility).
The impressive capabilities of the 10x Genomics 5,000-plex human gene expression panel were demonstrated using the Xenium Analyzer system, which successfully imaged a total area of 420 mm² over 124 hours. A deep analysis of a human liver sample slide revealed an average of 408 transcripts per cell across 1,018,026 cells, with a low false discovery rate of just 0.3%. This detailed examination identified 26 distinct cell types and five unique spatial niches, confirmed by visualizing marker genes for accurate cell type and neighborhood assignments. The 5,000-plex panel offers comprehensive coverage, making it an excellent tool for classifying cell types, exploring drug target interactions, investigating cell-cell signaling, and mapping biological pathways across all human tissues.
Alex Rolland and his team at Cancer Treatment Options and Management Inc. focus on advancing oncology research to enhance cancer diagnostics and treatment effectiveness. Their collaboration with Norgen Biotek has yielded significant advancements in liquid biopsy technology, particularly in extracting circulating tumor DNA (ct-DNA) and exosomal RNA from patient blood samples. By utilizing Norgen's innovative extraction kits, they achieved high yields of cancer-specific ct-DNA fragments, crucial for monitoring treatment responses and disease progression. Their approach includes custom probes to monitor treatment responses and detect mutations, crucial for guiding personalized therapies and improving patient outcomes worldwide.
