The Thermo Scientific NanoDrop One C Spectrophotometer is a compact, stand-alone UV-visible spectrophotometer for measurement of highly concentrated samples across a wide variety of analytes without the need for dilution. In addition, the device is equipped with standard cuvette to measure dilute samples and optical density of bacterial cultures or to perform kinetics experiments. A glove-compatible touchscreen offers easy access to preloaded software applications and enables purity and concentration assessments in seconds. Built-in Acclaro Sample Intelligence technology helps identify and correct for the contaminants present. Make informed decisions on sample quality and improve your success in downstream applications with NanoDrop technology.
General information: NanoDrop One C
| Product Name | NanoDrop™ One C |
| Origin | US |
| Brand | Thermo Fisher Scientific |
| Authorized distributor in Vietnam | GeneSmart Co., Ltd |
| Reference information | Brochure: NanoDrop Family
Application note: Monitoring enzyme kinetics with the NanoDrop OneC Spectrophotometer |
Our flagship NanoDrop One C spectrophotometers share common features with other members of the NanoDrop family
- Requires only 1–2 µL of sample without dilution, even for highly concentrated samples
- Simply pipet a drop of your DNA, RNA, or protein sample on the pedestal and pull down the arm; the NanoDrop OneC instrument includes a built-in cuvette capability. The cuvette position adds experimental flexibility and increases the dynamic range. Combining the pioneering sample-retention system with cuvette capability accommodates both low and high concentrations (0.2–27.500 ng/µL dsDNA and 0.006–820 mg/mL BSA). Use cuvettes to measure dilute samples and optical density of bacterial cultures or to perform kinetics experiments. The cuvette position can be used with the arm up or down and includes temperature control and stirring features.
- Results appear in seconds of initiating a measurement, and Auto-Blank and Auto
- Measure settings can further streamline your workflow
- Measure up to 27.500 ng/µL dsDNA or 400 mg/mL IgG without dilution
DNA, RNA, and protein quantification, full spectral data, purity ratios (A260/A280, A260/A230), and more - Measure the full UV-Vis spectrum from 190–850 nm, allowing a range of assays as:
+ Peptides (205 nm)
+ DNA and RNA (260 nm)
+ Purified protein (280 nm)
+ Toxicology assays and industrial dyes (490 nm)
+ Gold nanoparticles (520 nm)
+ Colorimetric protein assays (BCA 562 nm, Bradford 595 nm, Modified Lowry 650 nm, Pierce 660 660 nm)
+ Optical Density measurements (600 nm)
NanoDrop One C Highlights
- Contains both pedestal and cuvette positions to expand experimental flexibility and increase the dynamic range.
- Measure dilute samples, perform kinetics experiments and take optical density measurements of bacterial cultures.
- Cuvette position includes temperature control and stirring.
Acclaro Sample Intelligence Technology
Thermo Scientific Acclaro Sample Intelligence Technology software analyzes absorbance across the whole UV-Vis spectrum, not just wavelengths specific to nucleic acids and proteins. Determine absorbances from known contaminants like protein or phenol in dsDNA or RNA samples or nucleic acids or phenol in protein samples. Detect the presence of guanidine HCl and guanidinium isothiocyanate in nucleic acid samples, dsDNA in RNA, or RNA in dsDNA. It then applies powerful chemometric algorithms to identify contaminants in your samples and correct quantitation results. You get more reliable downstream assay results and avoid potential rework with this efficient, breakthrough capability.
Stand-alone instrument with local control and Built-in touch screen
- Auto-blank and auto-measure option
- Sample volume 1.0 – 2.0 microliter – direct measurement or measurement with cuvette .
Detection Range: Pedestal: 2 ng/μL (dsDNA), 0.06 mg/mL (BSA); Cuvette: 0.2 ng/μL (dsDNA), 0.006 mg/mL (BSA) - Maximum Concentration: Pedestal: 27,500 ng/μL (dsDNA), 820 mg/mL (BSA); Cuvette: 75 ng/μL (dsDNA)
- Measurement and Data Processing Time: 8 seconds
- Repeatability: Typical: 0.002 A (1.0 mm path) or 1%CV, whichever is greater (SD of 10 individual measurements at 0.97 A)
- Wavelength Range: 190–850 nm
- Wavelength Accuracy: ±1 nm
- Photometric Range (10 mm equivalent): Pedestal: 0–550 A; Cuvette: 0–1.5 A
- Photometric Accuracy: 3% at 0.97A, 302 nm, (Absorbance expressed at Abs/mm at 25°C)
- Spectral Bandwidth: ≤1.8 nm (FWHM at Hg 254 nm)
- Pathlength: 0.030 to 1.0 mm (auto-ranging)
- Light Source: Xenon flash lamp
- Detector: 2048-element CMOS linear image sensor
- Dimensions: 20 x 25.4 x 32.3 cm (8 x 10 x 12.7 in) (L x W x H)
Weight: 3.6 kg (7.9 lb) - Voltage: 12 VDC
- Power Consumption: Operating: 12 to 18W; Standby: 5W
- Languages: English, French, German, Spanish, Polish, Chinese, Japanese, Korean
Acclaro™ Sample Intelligence technology
Acclaro technology enhances user understanding of sample quality while delivering accurate quantitative measurements. Early identification of contaminants can prevent failure of downstream applications and save days of troubleshooting.
- Verify sample quality in seconds – identify sample contaminants and obtain corrected concentration results.
- Access information through alerts – receive instant feedback about sample quality with on-demand technical support and guided troubleshooting.
- Rely on accurate results – ensure measurement integrity with embedded sensor and digital image analysis.
- Identify nucleic acid contaminants such as RNA contamination in dsDNA samples and chemical contaminants such as phenol in RNA samples
NanoDrop One C PC Software with 21 CFR Part 11 Compliance
- New PC Control software allows users to control the instrument from an Ethernet connected PC
- Optional Thermo Scientific Security Suite for NanoDrop One C allows the instrument to be used in a 21 CFR Part 11 compliant lab. Features include: User account access, Audit trail, Electronic signatures, Automated data back-up, and more
NanoDrop One C Enhancements
- Ergonomic, standalone design – integrated Android™ tablet eliminates the need for a separate computer and provides seamless data transfer via Wireless*, Ethernet or USB.
- Auto-Measure and Auto-Blank functions – multi-sample processing can be streamlined with instant measurements that occur when the pedestal arm is down. These functions can be turned ON or OFF with a single tap on the screen.
- Wider dynamic range – no dilutions required for highly concentrated samples (dsDNA at 27,500 ng/μL) with auto-range pathlength technology
- Integrated Learning Center – an archive of technical support documents and educational animations at your fingertips.
Advanced Connectivity for Data Storage, Printing and Sharing
- Multiple choices for data export: transfer results with a USB, Wi-Fi or Ethernet connection to your PC or save data directly to the Thermo Fisher Cloud via Wi-Fi or Ethernet network.
- View, organize and share data on a PC: Once exported, you can view the full-data formats (e.g., contaminant analysis spectral graphs) and organize experiments using the NanoDrop One C PC Control software.
- Easy data-to-printer options: Print your results directly from the NanoDrop One C instrument via Wi-Fi, Ethernet or USB printer connection; print exported data from your PC using the Nanodrop One C PC Control software.
- 24/7 access to data: View data anytime, anywhere from any device (Android-based or iOS-based devices, PC or Mac desktop computers) when you collect, analyze, store and share your NanoDrop One data on the NanoDrop App found on the [https://www.thermofisher.com/us/en/home/cloud.html] Thermo Fisher Cloud. Requires registration.
- Online collaborations: Share data between colleagues who do not have an instrument and view NanoDrop One C data across multiple Thermo Fisher Cloud-connected accounts.
