With extensive expertise and state-of-the-art bioinformatics pipelines, Novogene’s shotgun metagenomic sequencing services provide researchers with the tools to explore microbial genomes, functions, and genetic variations across diverse sample types. Our solutions ensure high-quality data, publication-ready results, and customized analyses tailored to meet a wide range of research needs. From characterizing community structures and classifying species to investigating system evolution, gene functions, and metabolic networks in environmental microbiomes, Novogene delivers comprehensive metagenomics solutions to drive scientific discovery.
Applications
- Shotgun metagenomic sequencing offers a versatile platform for a broad range of research applications, encompassing taxonomic profiling, functional analysis, and metabolic network studies. Key applications include:
- Comprehensive analysis of microbial communities, providing insights into their taxonomic and functional profiles.
- Detection of micoorganisms relevant to human health, environmental remediation, and sustainable energy production.
- In-depth exploration of microbial genetic blueprints and metabolic pathways to inform pharmaceutical innovation.
- Investigation of the complex relationshisp between microbes and their hosts, facilitating advancements in drug discovery and therapeutic developments.
- These applications highlight the utility of shotgun metagenomic sequencing in driving discoveries across diverse scientific disciplines.
Benefits of Shotgun Metagenomic Sequencing:
- Shotgun metagenomic sequencing enables simultaneous analysis of multiple microorganisms without isolation, cultivation, or target region amplification, providing insights into microbial and functional diversity across prokaryotes and eukaryotes.
- With extensive experience in metagenomic sequencing, Novogene delivers high-quality results for diverse sample types, including soil, fecal, and water.
- Novogene provides a wide range of analysis options tailored to your research needs, including assembly-based analysis and two distinct read-mapping approaches for comprehensive data interpretation.
Specifications: DNA Sample Requirements
| Sample Type | Amount | Volume | Concentration | Purity |
| Total DNA | ≥ 100 ng | ≥20 μL | ≥ 5 ng/μL | OD260/280 = 1.8-2.0, No degradation, no contamination, no color |
Specifications: Sequencing and Analysis
| Sequencing Platform | Illumina NovaSeq Platforms | |
| Sequencing Strategy | Pair-end 150 bp | |
| Recommended Sequencing Depth | ≥ 6Gb for simple environments
≥ 12Gb for complex environments |
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| Analysis Options (Choose among these three) |
Standard Analysis(Assembly-based) | Data quality control (host contamination removal if required)
Metagenome Assembly Gene Prediction Taxonomy Annotation Function Annotation (KEGG, eggNOG, CAZY…etc) Alpha and Beta Diversity Analyses Antibiotic Resistance Gene and Mobile Genetic Elements Annotation and Analyses |
| MetaPhlAn4-HUMAnN3(Reads-Mapping Based) | Data quality control (host contamination removal if required)
Taxonomy Annotation Function Annotation ( MetaCyc, KEGG, eggNOG, GO…etc) Alpha and Beta Diversity Analyses Differential analysis between groups of species and function. |
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| Kraken2(Reads-Mapping Based) | Data quality control (host contamination removal if required)
Taxonomy Annotation Function Annotation ( KEGG, eggNOG, GO…etc) Alpha and Beta Diversity Analyses Differential analysis between groups of species and function. |
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Project Workflow
Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, fragmentation and library preparation, library quality control, sequencing, and bioinformatics analysis.
