Novogene provides end-to-end service processes including DNA extraction, library preparation, sequencing, and analyses for Mouse Whole Exome Sequencing. These services empower researchers to identify causative mutations in mice with distinguishable phenotypes, thus discovering genes associated with human diseases and clarifying drug resistance evolution mechanisms in mouse models.
Applications
- Mouse Whole Exome Sequencing has been helping researchers in elucidating the following mechanisms:
- Drug development in cancer treatment
- Model research on human health and disease
Benefits
- Agilent SureSelectXT Mouse Exon Kit and NovaSeq 6000 platforms are available for mWES service.
- Data quality with a guarantee of Q30≥85%.
- Average coverage of target region with a percentage of 99.6+/- for mWES.
- Ready-for-publication data and figures bring insights for result overview based on Novogene’s cutting-edge bioinformatics pipeline and database.
Specifications: Sample Requirements
| Sample Type | Amount (Qubit®) | Purity |
| Genomic DNA | ≥ 300 ng | A260/280=1.8-2.0;
no degradation, no contamination |
| Genomic DNA from FFPE tissue | ≥ 500 ng | Fragments should be longer than 1000 bp |
Specifications: Sequencing and Analysis
| Platform | Illumina NovaSeq 6000 |
| Read length | Paired-end 150 bp |
| Sequencing depth | Effective sequencing depth above 50× (6G) |
| Standard data analysis | Data Quality Control
Alignment with reference genome, statistics of mapping, depth sequencing depth and coverage SNP and InDel calling, annotation and statistics Somatic SNP/InDel/CNV calling, annotation and |
Project Workflow
