Novogene offers comprehensive end-to-end single-cell sequencing services, including Single Cell 3′ Gene Expression, Single Cell 5′ Gene Expression, Single Cell Immune Profiling, and Single Cell ATAC, etc. Our services provide comprehensive support for your projects, from experimental design consultation, onsite services*, library preparation, Next Generation Sequencing, and bioinformatics analysis reports.
As a Certified Service Provider of 10x Genomics and Illumina NGS platforms, you can trust the highest quality and reliability in our single-cell sequencing workflows.
*On-site single cell capture service is available to customers in Singapore and Japan only.
Applications
- Single Cell Gene Expression: Profiling of cellular heterogeneity, novel targets, and biomarker in each cell from a heterogeneous cell population.
- Single Cell Immune Profiling: Analyze full-length, paired B-cell or T-cell receptors, antigen specificity, and gene expression, all from a single cell.
- Single Cell ATAC: Analyze chromatin accessibility at the single-cell level, providing insights into cell types and states, and deeper understanding of gene regulatory mechanisms*
*Do not provide single cell ATAC multiome service.
Sample Requirements
| Sample Type | Service | Sample Amount | Cell Viability |
| Fresh single cell suspension Single nuclei suspension* |
On-site service* | Single Cells ≥ 1,000,000 | Viability ≥ 80%, Diameter: 5-30 μm |
| Frozen single cell suspension** | Shipping with dry ice | Single Cells ≥ 1,000,000 | Viability ≥ 80%, Diameter: 5-30 μm |
* On-site service applies to Singapore and Japan customer only. Single nuclei suspension is available for on-site service only.
** Please download and complete the form if you are sending frozen single cells to us.
Specifications: Sequencing & Analysis
| Sequencing Platform | NovaSeq 6000 or NovaSeq X Plus | |
| Read length | Pair-end 150bp | |
| Recommended Data | 20,000 – 50,000 read pairs/cell, around 120Gb/sample | |
| Analysis | Cell Ranger Analysis | Standard Analysis |
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Project Workflow
We support an end-to-end suite of services to ensure that our clients gets the best quality data – This includes a pre-project consultation with our application experts in experimental design, subsequent sample preparation, library construction, sequencing, data quality assurance, and bioinformatics analysis.
Our standard analysis package encompasses the identification of distinct cell populations and differential gene expression, equipping researchers with in-depth insights to drive their scientific discoveries forward. Leveraging the Cell Ranger analysis pipeline, we expertly manage raw sequencing data through meticulous quality control, precise alignment, and accurate quantification processes, transforming complex data into actionable insights.
Our experience spans an impressive array of over 300 species and sample types, encompassing a broad spectrum of biological systems from well-established model organisms to the rare and unique, such as the Siberian Tiger, Turtles, and Lungfish. This extensive experience ensures that we deliver robust and reliable results.
