Applications
Cancer Research: Decode tumor cell heterogeneity and the tumor microenvironment at single-cell resolution, paving the way for groundbreaking advancements in cancer prevention, diagnosis, and treatment.
Neuroscience: Reconstruct three-dimensional organ structures and quantify spatiotemporal dynamics, shedding light on neural connectivity and brain function.
Developmental Biology: Map cell type interactions and molecular pathways during development with unprecedented precision, advancing our understanding of developmental mechanisms.
Sample Requirements
| Sample Type | Sample Amount | Storage Conditions | Sample QC | Shipping |
| FFPE Tissue Block |
Sample thickness > 1mm, area < 9mm x 9mm (standard chip) | Recommended for samples within 3 years; after embedding, store at 4°C |
DV200≥30% | 4°C or Room Temperature |
| FFPE Tissue Section |
10-15 FFPE(5μm thickness) scrolls in 1.5mL tube for sample QC； 4-6 FFPE(5μm thickness) tissue sections, place each FFPE sections into a new 50 mL centrifuge tubes for library prep |
After embedding, store at 4°C; RNA QC samples: stored in dry EP tubes; Formal samples: vertical in slide boxes, avoid contact between slides |
DV200≥30% | 4°C or Room Temperature |
Specifications:
| Stereo-FFPE | |
| Capture method | Random probe capture |
| Resolution | 500nm |
| Sequencing platform | BGI DNB-SEQ T7 |
| Read length | PE 25bp+59bp |
| Recommended Data Amount | 2.5G read pairs/210Gb/1cm2 |
| Capture Area | 1cm*1cm |
| Analysis | Data QC
Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG) DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment |
Project Workflow
