As protein-DNA complexes are recovered from living cells, binding sites can be compared in different cell types and tissues, or under different conditions. At Novogene, we offer high-quality sequencing and comprehensive bioinformatics solutions for your ChIP-seq projects.
Applications
ChIP-seq provides regulatory solutions and meets customized research needs, such as:
- Understanding how transcription factors regulate genes by profiling a genome-wide binding site and analyzing motif
- Outlining histone modification patterns associated with experimental treatment or sample conditions
- Investigating the relationships between epigenetic profiles and transcriptional regulations through joint analysis
- Accommodating to multiple immunoprecipitated DNAs using various methods, including ChIP, Cut&Tag, etc..
Benefits
- The output data is stable and reliable, with the guaranteed data quality of the sequencing score Q30≥ 80%.
- Expert bioinformatics analysis utilizes industry-standard MACS2 software and programs with the latest version to get motif prediction, peak annotation, functional analysis, and data visualization.
- Associated analysis is provided to explore correlations between ChIP-seq and gene expression.
Specifications: DNA Sample Requirements
| Sample Type | Required Amount | Fragment size | Purity |
| Enriched DNA Sample | ≥ 20 ng (Concentration ≥ 2 ng/μL) | 100 bp~500 bp | A260/280=1.8-2.0 |
Specifications: Sequencing & Analysis
| Platform | Illumina NovaSeq 6000 |
| Read Length | Pair-end 150 bp |
| Sequencing Depth | ≥ 20 million read pairs per sample for the species with reference genome |
| Standard Data Analysis |
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Project Workflow
Novogene ChIP-seq service comprises four steps, including sample preparation, DNA library preparation, Illumina PE150 sequencing and data analysis using bioinformatics pipelines. From DNA sampling to obtaining data reports, each step may affect the quality and quantity of data output, directly affecting the results of subsequent bioinformatics analysis. Novogene strictly checks every step, including sample quality control, library quality control, and sequencing data quality control, to ensure the high quality, accuracy and reliability of sequencing data and provide comprehensive bioinformatics analysis.
