Novogene provides whole genome bisulfite sequencing (WGBS) to efficiently identify methylated cytosines on a genome-wide scale with a single nucleotide resolution. The deep reach of the methylome is imperative for understanding gene expression and multiple biological processes that are subject to epigenetic regulation.
Applications
- Novogene delivers high-quality data and publication-ready analysis results of WGBS services, to faciliate research including:
- Profiling methylation patterns at CG, CHG and CHH sites with single nucleotide resolution
- Identifying differentially methylated sites associated with experimental treatments or sample conditions
- Understanding mechanisms of cell differentiation or tissue development on the basis of methylation profiles
- Early diagnosis of diseases and cancers through the detection of DNA hypermethylation or hypomethylation
Benefits
- Hundreds of projects have been successfully completed with highly professional, publication-quality data output and bioinformatics analysis.
- Ultra-low input DNA and high bisulfite conversion rate are available in methylation library preparation.
- Industry-standard software (such as Bismark) and mature in-house pipelines are used for mapping and comprehensive bioinformatics analysis.
- Customized association analysis can explore correlations between methylation and gene expression.
Specifications: DNA Sample Requirements
| Sample Type | Required Amount | Purity |
| Genomic DNA | ≥ 200 ng | A260/280=1.8-2.0 |
Specifications: Sequencing and Analysis
| Platform Type | Illumina NovaSeq 6000 |
| Read Length | Paired-end 150 bp |
| Sequencing Depth | ≥ 30× coverage for the species with reference genome |
| Standard Data Analysis | Data Quality Control
mCs detection, methylation level calculation Methylation level and frequency distribution Differentially Methylated Site (DMS) detection Differentially Methylated Regions (DMRs), Differentially Methylated Promoter (DMPs) detection and annotation Function enrichment of DMR-associated genes and DMP-associated genes Visualization of BS seq data Comparative analysis (among samples) |
Project Workflow
Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, library preparation, library quality control, sequencing, and bioinformatics analysis.
