Novogene offers Animal and Plant Whole Genome Sequencing services with ultra-fast turnaround time, high-quality sequencing data, and reliable results. Animal and Plant Whole genome sequencing has found its applications in several fields including population genetics research, genome-wide association studies (GWAS), and agricultural breeding programs.
Applications
- Reveals the molecular mechanism referring to breeding and speciation
- Provides resources for accelerating genetic development
- Investigates the origin and evolution of species
- Identifies common genetic variations among populations
Benefits
- Strict SOP is followed to ensure express project delivery.
- Unsurpassed data quality with Q30≥85%.
- Customized and advanced solutions are available for population and Genome-wide association study.
- Extensive experience with a wide range of plants and animals such as pigs, mice, tigers and bees.
Specifications: DNA Sample Requirements
| Platform Type | Sample Type | Amount (Qubit®) | Purity |
| Illumina
NovaSeq X Plus /NovaSeq6000 |
Genomic DNA | ≥ 200 ng | A260/280=1.8-2.0;
no degradation, |
| Genomic DNA
(PCR free non-350bp) |
≥ 5 μg | ||
| Genomic DNA
(PCR free -350bp) |
≥ 1.2 μg | ||
| PacBio Sequel II DNA CLR library |
HMW Genomic DNA | ≥ 8 μg | A260/280=1.8-2.0; A260/230=1.5-2.6; *NC/QC=0.95-3.00 Fragments should be ≥ 40 kb |
| PacBio Revio/sequel II/sequel IIe DNA HiFi library | HMW Genomic DNA | ≥ 5 μg | A260/280=1.8-2.0; A260/230=1.5-2.6; *NC/QC=0.95-3.00 Fragments should be ≥ 30 kb |
| Nanopore PromethION | HMW Genomic DNA | ≥ 8 μg | A260/280=1.8-2.0; A260/230=1.5-2.6; Fragments should be ≥ 30 kb |
*NC/QC: NanoDrop concetration/Qubit concentration
Specifications: Sequencing and Analysis
| Platform Type | Illumina NovaSeq X Plus /NovaSeq6000 | PacBio Revio/sequel II/sequel IIe | Nanopore PromethION |
| Read Length | Paired-end 150 bp | Average > 15 kb | Average > 17 kb |
| Recommended
Sequencing Depth |
For SNP/InDel detection: ≥ 10× | For SV detection: ≥ 20× | |
| For SV/CNV detection: ≥ 20× | |||
| Content of
Analysis |
Standard Analysis
Data quality control Sequencing error rate Filtering reads containing adapter or with low quality Alignment with reference genome Statistics of mapping, sequencing depth and coverage SNP calling, annotation and statistics Advanced Analysis SV calling, annotation and statistics CNV calling, annotation and statistics |
Standard Analysis
Data quality control Sequencing alignment Structural Variant (SV) detection |
|
Project Workflow
From sample and library preparation, short and long-read sequencing, and data quality control, to bioinformatics analysis, Novogene provides high-quality products and professional services. Each step is performed in agreement with a high scientific standard and meticulous design to ensure high-quality research results.
