With extensive experience and well-developed bioinformatics know-how, Novogene delivers high-quality data, publication-ready analysis figures, and personalized results to meet different research objectives and customer needs. Novogene offers ultra-fast turnaround time, even for large projects: equipped with numerous Illumina NovaSeq X Plus /NovaSeq6000 platforms, Oxford Nanopore PromethION and PacBio Revio/Sequel II system.
Novogene is capable of sequencing up to 200,000 human genomes per year at a competitive cost. Novogene hWGS service can provide data across a broad range of applications, including studies on genetic diseases, cancers, pathogenesis mechanisms, or population genetics. Multiple DNA sequencing technologies available at Novogene can identify the highly polymorphic and highly repetitive regions within the genome of interest, thereby providing a complete and accurate human genome characterization [1].
Applications
Human genome sequencing has been assisting researchers in the following research areas:
-
- Genetic diseases
- Cancers
- Pathogenesis mechanisms
- Human population origins
Advantages
Short-read sequencing
-
-
- Efficient discovery and documentation of genomic variants such as SNPs, InDels, SVs and CNVs.
- Cost-effective strategy, low-pass sequencing, and imputation services for genome-wide association studies (GWAS) for larger cohorts or complex disease research.
-
Long-read sequencing
-
-
- Efficient discovery of repeated sequences and large structural variants.
- Direct detection of methylation information (5mC in CpG sites).
-
Specifications: DNA Sample Requirements
| Platform Type | Sample Type | Amount (Qubit®) | Purity |
|
Illumina
NovaSeq X Plus /NovaSeq6000 |
Genomic DNA | ≥ 200 ng |
A260/280=1.8-2.0;
no degradation,
no contamination |
| Genomic DNA (PCR free) |
≥ 1.2 μg | ||
| Genomic DNA from FFPE tissue |
≥ 400 ng | Fragments longer than 1,000 bp | |
| PacBio Revio/ Sequel II system HiFi library | HMW Genomic DNA | ≥5 μg | A260/280=1.75-2.0; A260/230=1.4-2.6; *NC/QC=0.95-3.00Fragments should be ≥ 30 kb |
| Nanopore PromethION |
HMW Genomic DNA | ≥ 8.5 μg | A260/280=1.75-2.0; A260/230=1.4-2.6;Fragments should be ≥ 30 kb |
*NC/QC: NanoDrop concentration/Qubit concentration
Specifications: Sequencing and Analysis
| Platform Type | Illumina NovaSeq X Plus /NovaSeq6000 | PacBio Revio/ Sequel II system | Nanopore PromethION |
| Read Length | Paired-end 150 bp | > 15 kb (Average) |
> 17 kb (Average) |
|
Sequencing Depth
|
For rare diseases: 30-50× |
For genetic diseases: 10-20× |
For genetic diseases: 10-20× |
| For tumor tissues: 50×; For adjacent normal tissues and blood: 30× |
For tumor tissues: ≥20× |
For tumor tissues: ≥20× |
|
|
Standard Data
Analysis |
|
|
|
Note: Values of sequencing depths are only listed for your reference. For more information, please contact us.
Project Workflow
From sample preparation, library preparation, DNA sequencing and data quality control, to bioinformatics analysis, Novogene provides high-quality products and professional services. Each step is performed in agreement with a high scientific standard and meticulous design to ensure high-quality research results.
[1] https://www.genome.gov/about-genomics/fact-sheets/Sequencing-Human-Genome-cost
