The QuantSeq REV V2 Kit is an all-in-one library preparation protocol designed to generate Illumina compatible libraries of sequences close to the 3’ end of polyadenylated RNA.
With this kit the exact 3’ end can be pinpointed as the NGS reads directly start from the poly(A) tail using a Custom Sequencing Primer (CSP) which is included in the kit. This version of the kit is also recommended for paired-end sequencing.
For multiplexing pre-mixed UDIs of the Lexogen UDI 12 nt Unique Dual Indices (UDIs) are included in the kit. Up to 384 UDIs are compatible with QuantSeq REV. For multiplexing needs beyond 384 samples.
QuantSeq Kits are provided with codes for free gene expression data analysis on Lexogen’s automated data analysis solution. For more information, please visit Lexogen’s data analysis solutions.
Kit Contents:
- Library Generation Module including the Custom Sequencing Primer
- Library Amplification Module
- Lexogen 12 nt Unique Dual Indexing Set B1 with UDI12B_0001-0024 or UDI12B_0001-0096 depending on the kit size (24, 96)
- Purification Module with Magnetic Beads
System Compatibility:
Illumina:
- HiSeq 2000/2500/3000/4000
- NextSeq 500/550 (with CSP Version 5)
- NovaSeq 6000 / NovaSeq X
- MiSeq
- iSeq 100
- MiniSeq
Element AVITI*
Singular Genomics G4*
*mixed lane with other Lexogen libraries, sequenced without CSP.
Product Items
| 225.24 | QuantSeq 3’ mRNA-Seq V2 Library Prep Kit (REV) with Custom Sequencing Primer and UDI 12 nt Set B1, 24 preps |
| 225.96 | QuantSeq 3’ mRNA-Seq V2 Library Prep Kit (REV)with Custom Sequencing Primer and UDI 12 nt Set B1, 96 preps |
