With extensive experience in experimental operations and bioinformatics analyses, Novogene offers an accurate, rapid, and comprehensive characterization of species and generates reliable results. Furthermore, Novogene’s end-to-end services guarantee you ultra-fast turnaround time.
Applications
For individual research：
- Guides animal health and genetic breeding
- Provides a theoretical basis for drug screening
- Explores medicinal resources and innovates varieties
For population research：
- Explores species origin and evolution
- Provides new insights into patterns of genome divergence
Applications
For individual research：
- Guides animal health and genetic breeding
- Provides a theoretical basis for drug screening
- Explores medicinal resources and innovates varieties
For population research：
- Explores species origin and evolution
- Provides new insights into patterns of genome divergence
Benefits
Highly experienced: Novogene’s highly qualified researchers have completed major De novo genome sequencing projects and managed to publish their data in top-tier journals.
Bioinformatics expertise: Best-in-class and widely recognized software, such as Falcon and Canu, are being used for comprehensive plant and animal bioinformatic analyses.
Diverse strategies: By incorporating sequencing results from various platforms including Illumina Novaseq, PacBio Revio/Sequel II/Sequel IIe, and Oxford PromethION, we offer the best assembly solution specifically tailored for each unique genome.
Unsurpassed data quality: We guarantee a Q30 score ≥ 85%, exceeding Illumina’s official guarantee of ≥ 75%.
Specifications: DNA Sample Requirements
| Platform Type | Sample Type | Amount (Qubit®) | Purity |
| Illumina NovaSeq 6000 |
Genomic DNA | ≥ 200 ng | A260/280=1.8-2.0; no degradation, no contamination |
| Genomic DNA (PCR free non-350bp) |
≥ 5 μg | ||
| Genomic DNA (PCR free -350bp) |
≥ 1.2 μg | ||
| PacBio Sequel II DNA CLR library |
HMW Genomic DNA | ≥ 5 μg (Concentration ≥ 70 ng/μL) |
A260/280=1.75-2.0; A260/230=1.5-2.6; NC/QC=0.95-3.00 Fragments should be ≥ 30 kb |
| PacBio Revio/sequel II/sequel IIe DNA HiFi library | HMW Genomic DNA | ≥ 15 μg (Concentration ≥ 70 ng/μL) |
A260/280=1.8-2.0; A260/230=1.5-2.6; NC/QC=1.00-2.20 Fragments should be ≥ 30 kb |
| Nanopore PromethION | HMW Genomic DNA | ≥ 8 μg (Concentration ≥ 100 ng/μL) |
A260/280=1.75-2.0; A260/230=1.4-2.6; NC/QC=0.95-3.00 Fragments should be ≥ 30 kb |
NC/QC:NanoDrop concetration/Qubit concentration
Specifications: Sequencing and Analysis
| Sequencing Parameters | Illumina Novaseq 6000 | PacBio Revio/sequel II/sequel IIe | Nanopore PromethION |
| Read Length | Paired-end 150 bp | N50>15 kb, long read lengths up to 25 kb（CCS） | average > 17 kb |
| Recommended Sequencing Depth | For genome survey or assembly polishing: ≥ 50× | For genome assembly: ≥ 50× | |
| Standard Analysis | K-mer analysis
GC content analysis Repeat content rate evaluation Heterozygous rate evaluation Genome size evaluation |
Long-read assembly
Assembly Statistics Gene completeness evaluation |
|
| Genome Annotation | － | Repeat prediction
Structure prediction Function prediction Noncoding RNA prediction |
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Project Workflow
From sample preparation library preparation, short and long-read sequencing, and data quality control, to bioinformatics analysis, Novogene provides high-quality products and professional services. Each step is performed in agreement with a high scientific standard and meticulous design to ensure high-quality research results.
