Iso-Seq can be fully leveraged for medical and agricultural research purposes, including disease mechanism investigation, exploring drug resistance mechanisms, discovering new genes, as well as studying plant development and biotic and abiotic stresses
Applications
- For investigating disease mechanisms
- For discovering alternatively spliced transcripts as potential biomarkers
- For exploring drug resistance mechanisms
- For identifying new genes and transcripts
- For improving genome annotations to recognize the coding regions, regulatory elements, and structural elements of the genes
- For understanding plant development under environmental stress
- For discovering new isoforms resulting from alternative splicing
Benefits
- Leading sequencing capacity, high-quality data, fast turnaround, and affordable prices.
- Well-developed pipeline to discover novel transcripts and perform differential expression and functional annotation analyses.
- Ability to optimize the sequencing process to surpass PacBio’s standards in read length and output.
Kinnex Full-length RNA Kit
The Kinnex kits are based on the MAS-Seq method, which concatenates smaller amplicons into larger fragment libraries for throughput increase. Novogene uses the Kinnex full-length RNA kit and PacBio Revio system, as part of its isoform sequencing pipeline to further improve the throughput. This enables large-scale studies at a resolution difficult to attain with short-read RNA sequencing.
Specifications: RNA Sample Requirements
| Library Type | Sample Type | Amount | Concentration | RIN (Agilent 2100) |
Purity (Nanodrop TM/Agarose Gel) |
| PacBio Kinnex full-length RNA library | Total RNA | ≥ 600 ng | ≥ 40 ng/μL | ≥ 6.5 | A260/280= 1.8-2.2; A260/230= 1.3-2.5; *NC/QC≤2 |
*NC/QC: NanoDrop concentration/Qubit concentration.
Specifications: Sequencing and Analysis
| Platform | PacBio Revio |
| Library Type | Kinnex full-length RNA library |
| Recommended data amount | 5M/ 10M HiFi reads per sample |
| Content of analysis | Data QC
Function Annotation With reference genome Structure analysis Alternative splicing Alternative polyadenylation Novel gene/ Novel transcript prediction and annotation Transcription factor analysis LncRNA prediction Fusion transcript analysis Without reference genome Structure analysis Prediction of coding sequences Transcription factor analysis SSR analysis LncRNA prediction Quantification analysis is available without the aid of short-read mRNA-seq when choosing 10M HiFi reads with Kinnex full-length RNA library. Gene/ Transcript expression level analysis Differentially expressed gene/ transcript analysis (Transcript analysis can only be conducted when a reference genome is available.) |
Project Workflow
Novogene provides high-quality products and expert services throughout the entire project workflow. Every step is carefully designed and executed to meet rigorous scientific standards, ensuring exceptional research outcomes. To guarantee the accuracy and reliability of sequencing data, stringent quality control (QC) measures are implemented at each stage of the process. The workflow encompasses key steps such as sample preparation and quantification, library preparation, library quality control, sequencing, and bioinformatics analysis.
