THÔNG TIN MÔ TẢ
Maximize your insights: Gain a deeper, more complete assessment of your samples with protein, mRNA, and morphology from the same tissue section.
Profile entire tissue sections: Visualize protein detection and whole transcriptome expression, without the need to select regions of interest.
Streamlined data analysis: Combine protein detection profiles with gene expression data in easy-to-use software.
Rich insights
- Single-section multiomics – Study both protein and RNA from a single tissue section using a curated, pre-validated antibody content panel
- Histology matters – The only multiplex protein assay that allows you to image an H&E or IF-stain before other chemical treatments
- Bias-free discovery – Unbiased gene expression analysis with whole transcriptome detection and no need for ROI selection with predetermined biomarkers
FAQ
What is Visium CytAssist Gene and Protein Expression?
How can I use Protein Expression data to better understand my tissue of interest?
Our Visium CytAssist Gene and Protein Expression solution allows for researchers to study the relative expression patterns of proteins within the Human Immune Cell Profiling Panel. An NGS read-out of Protein Expression data allows you to overcome challenges like spectral overlap and restricted antibody host species, which generally lead to limitations in the number of proteins that can be studied simultaneously in tissue. Furthermore, it enables users to interrogate immune protein markers that, typically, are lowly expressed as RNA.
What antibodies are included in the 10x Human Immune Cell Profiling Panel?
How do I analyze my Visium CytAssist Gene and Protein Expression data?
10x Genomics provides two types of software to help you analyze your data: Space Ranger and Loupe Browser. Space Ranger is an analysis software that automatically overlays spatial gene expression information on your tissue image and identifies clusters of spots with similar transcription profiles. You can then use Loupe Browser, a visualization software, to interactively explore the results. You can download Space Ranger and Loupe Browser from the 10x Genomics Support site at no cost.
Does the IF protocol combined with Visium deviate from standard IF protocols?
Yes, there are several key differences between standard IF and the IF protocol used with Visium. Please refer to the IF Demonstrated Protocol (FFPE | Fresh frozen) for additional details.
Does IF affect the Visium gene expression data?
Impact on Visium performance may vary from tissue to tissue due to the high sensitivity of the Visium assay. However, the spatial organization of gene expression and cell cluster detection is comparable between H&E and IF.
