THÔNG TIN MÔ TẢ
Choice: Start from FFPE blocks, or pre-sectioned FFPE or fresh frozen (FF) tissues on glass slides
Confidence: Pre-screen to find the most biologically relevant tissue sections.
Flexibility: Integrate seamlessly with standard histology workflows for H&E and IF staining.
Powerful platform for spatial discovery
The Visium CytAssist is a compact instrument designed to simplify the Visium workflow by facilitating the transfer of transcriptomic probes from standard glass slides to Visium slides, enabling whole transcriptomic spatial profiling insights across your entire tissue section, and expanded compatibility with FFPE and/or FF samples.
Simplified sample management
It all starts with a slide. The Visium CytAssist enables users to start with tissue on standard glass slides, allowing input tissue to be prepared, stained, and imaged using standard histology workflows.
Expanded sample access
Compatible with H&E or immunofluorescence stained FFPE or FF tissue sections, the CytAssist instrument allows you to use pre-sectioned and pre-stained tissues with 6.5 x 6.5 mm or 11 x 11 mm Visium Capture Area slides.
Prescreen tissue sections, and then use the instrument for precise manual alignment of tissue section onto Visium Capture Areas, enabling you to see more of the tissue you care about.
Visium workflow with CytAssist
Facilitate transfer of transcriptomic probes in FFPE or fresh frozen samples with Visium CytAssist. In the Visium CytAssist workflow, sectioning, tissue preparation, staining (H&E or IF), and imaging take place on a standard glass slide. After probe hybridization, two standard glass slides and a Visium slide with two Capture Areas are placed in the CytAssist instrument so that the tissue sections on the standard slides can be aligned on top of the Capture Areas. Within the instrument, a brightfield image is captured to provide spatial orientation for data analysis, followed by hybridization of transcriptomic probes to the Visium slide. The remaining steps, starting with probe extension, follow the standard Visium workflow outside of the instrument.
FAQ
How does CytAssist enable broader sample access?
Visium CytAssist enables access to fresh frozen sections or FFPE tissues archived in blocks or pre-sectioned onto standard glass slides. Existing samples can be stained with H&E staining or IF antibodies on the glass slides before introduction to the Visium CytAssist workflow.
What tissues can be used with CytAssist and the compatible Visium assays?
The CytAssist instrument uses kits with a probe-based chemistry compatible with human and mouse FFPE or fresh frozen samples.
Are there new kits required to run fresh frozen samples on CytAssist?
No. The existing Visium CytAssist Spatial Gene Expression for FFPE reagent and slide kits are compatible with both fresh frozen and FFPE tissue samples sectioned onto standard glass slides
