How can you ensure that your single cell RNA sequencing (scRNA-seq) studies have the best chance of success? As with so...
The Innovator Blog Series celebrates research conducted by 10x Genomics customers who have demonstrated scientific ingen...
The advent of single cell assays, such as 3'GEX and 5'GEX, has revolutionized our research in various fields like oncology, developmental biology, and immunology by enabling detailed gene expression analysis at a single-cell resolution. However, their reliance on fresh samples limited their application. Chromium Fixed RNA profiling- a highly sensitive probe-based technology- overcomes these challenges by allowing sample preservation and transport without the quality losses, simplifying logistics. It enhances efficiency by enabling sample batching and multiplexing. The technology also barcodes up to 1 million cells in 6 minutes, reducing hands-on time and errors. Additionally, its compatibility with FFPE tissues and integration with Visium and Xenium spatial solutions provides comprehensive disease insights, pushing the boundaries of innovation in research and beyond.
Those limitations are why our team developed this single cell RNA sequencing (scRNA-seq) protocol for PFA-fixed tissues. In this blog you’ll get a quick snapshot of how it offers you access to more samples, lets you process them on your schedule, retains your sample quality throughout storage and transport, and provides the greatest sensitivity of any commercial fixed RNA profiling protocol. Or, if you’re ready to jump right in, we have a direct link to the protocol and a webinar that goes into greater depth (and also offers a preview of FFPE tissue compatibility).
Cancer is a multifaceted disease driven by the complex interplay of diverse immune cells. To elucidate the heterogeneity of tumor microenvironments and assess the efficacy of innovative therapeutics, leveraging Chromium Single Cell Multiome ATAC + Gene Expression for comprehensive epigenetic profiling is essential. This advanced technique is pivotal in deciphering the mechanisms of gene activation and silencing that propel cancer progression.
10x Genomics has made a scientific breakthrough to resolve limitations of former methods when they introduced BEAM (Barcode Enabled Antigen Mapping).
Chromium Single Cell Immune profiling allows simultaneous analysis of T-cell receptor (TCR) sequences and gene expression profiles from the same individual cells, enabling researchers to measure clonal expansion and identify often overlooked immune cells in the tumor microenvironment.
Hematopoietic and pluripotent stem cells (HPSCs) mobilization is critical for effective autologous stem cell transplants...
Recent advancements have utilized the Chromium Single Cell Immune Profiling assay to develop an in-depth tumor microenvironment atlas, focusing on primary tumors, metastases, and particularly ascites – the fluid accumulation common in advanced cases. This study identified immune cells in ascites that could potentially enhance the immune response against primary and metastatic tumors.
Today, single cell RNA sequencing (scRNA-seq) is a well-established, reliable method enabling researchers to delve into...
