Single-cell research is opening up a revolutionary new avenue for understanding gene expression at the individual cell l...

Chromium Single Cell Immune profiling allows simultaneous analysis of T-cell receptor (TCR) sequences and gene expression profiles from the same individual cells, enabling researchers to measure clonal expansion and identify often overlooked immune cells in the tumor microenvironment.

10x Genomics has made a scientific breakthrough to resolve limitations of former methods when they introduced BEAM (Barcode Enabled Antigen Mapping). 

Those limitations are why our team developed this single cell RNA sequencing (scRNA-seq) protocol for PFA-fixed tissues. In this blog you’ll get a quick snapshot of how it offers you access to more samples, lets you process them on your schedule, retains your sample quality throughout storage and transport, and provides the greatest sensitivity of any commercial fixed RNA profiling protocol. Or, if you’re ready to jump right in, we have a direct link to the protocol and a webinar that goes into greater depth (and also offers a preview of FFPE tissue compatibility).

The Innovator Blog Series celebrates research conducted by 10x Genomics customers who have demonstrated scientific ingen...

How can you ensure that your single cell RNA sequencing (scRNA-seq) studies have the best chance of success? As with so...

Cell heterogeneity is a key contributor to biological complexity that is often masked by bulk techniques, such as RNA se...

Researchers from the University of Tokyo, University of Ryukyus, and Daiichi Sankyo Co. used single-cell technologies, including Chromium Single Cell ATAC and Gene Expression assays, to uncover the mechanisms of action and resistance of valemetostat, a dual inhibitor targeting EZH1 and EZH2, in treating adult T-cell leukemia/lymphoma (ATL). They found that valemetostat reduces H3K27me3 levels, leading to chromatin relaxation and reactivation of tumor suppressor genes, which improves patient outcomes. Additionally, the treatment increases expression at previously repressed gene loci, countering the epigenetic repression observed in cancer cells. By exploiting Chromium Single Cell ATAC, these findings open a new realm of therapy development, offering promising directions for more effective and durable cancer treatments.

Despite being a chronic, multifaceted autoimmune disease that can lead to several comorbidities, psoriasis has no standard treatment guidelines in Vietnam. Besides, it is also commonly mistaken for atopic dermatitis (AD) or clinically ambiguous rashes. This phenomenon raises the need to distinguish these diseases to improve diagnosis and prescribed treatments.By using single-cell technology, researchers have demonstrated that the presence of tissue-resident memory T-cell 1 (Trm1) is highly correlated with AD compared to psoriasis. An open-source web tool called RashX has been introduced, enabling researchers to upload 10x Genomics scRNA-seq immune cell dataset matrices and see how well their sample’s genetic signature aligns with other known AD or psoriasis samples. Although this tool is simple, it serves as a proof-of-concept for how single-cell research can contribute to precision medicine for the diagnosis and treatment of chronic inflammatory skin diseases.

The advent of single cell assays, such as 3'GEX and 5'GEX, has revolutionized our research in various fields like oncology, developmental biology, and immunology by enabling detailed gene expression analysis at a single-cell resolution. However, their reliance on fresh samples limited their application. Chromium Fixed RNA profiling- a highly sensitive probe-based technology- overcomes these challenges by allowing sample preservation and transport without the quality losses, simplifying logistics. It enhances efficiency by enabling sample batching and multiplexing. The technology also barcodes up to 1 million cells in 6 minutes, reducing hands-on time and errors. Additionally, its compatibility with FFPE tissues and integration with Visium and Xenium spatial solutions provides comprehensive disease insights, pushing the boundaries of innovation in research and beyond.

Cancer is a multifaceted disease driven by the complex interplay of diverse immune cells. To elucidate the heterogeneity of tumor microenvironments and assess the efficacy of innovative therapeutics, leveraging Chromium Single Cell Multiome ATAC + Gene Expression for comprehensive epigenetic profiling is essential. This advanced technique is pivotal in deciphering the mechanisms of gene activation and silencing that propel cancer progression.

Hematopoietic and pluripotent stem cells (HPSCs) mobilization is critical for effective autologous stem cell transplants...