HD Spatial Gene Expression

Whole transcriptome spatial discovery in high definition

Pair powerful whole transcriptome spatial gene expression with single cell–scale resolution to discover the unique biology in every micron of tissue. Obtain best-in-class data with the simple-to-run CytAssist-enabled workflow.

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THÔNG TIN MÔ TẢ

FFPE human prostate cancer tissue
Spatial discovery power at the resolution you need

Perform whole transcriptome gene expression analysis at single cell scale with continuous tissue coverage.

FFPE mouse embryo sample
Quality data from every micron of tissue

Leverage precise transcript localization enabled by Visium CytAssist to generate the highest quality spatial transcriptomics data.

Graphic of FFPE samples, including archived blocks and pre-sectioned slides
Access a broad range of FFPE samples

Generate powerful data from human or mouse FFPE samples, including archived blocks or pre-sectioned slides.

Graphic of stained tissue samples
Take histology to the next level

Enhance your H&E and IF imaged samples with high-resolution spatial transcriptomics from the same tissue section.

Visium HD slides

Next-generation slide architecture enables single cell–scale resolution.

Visium HD slide architecture

Visium HD Spatial Gene Expression slides contain two 6.5 x 6.5 mm Capture Areas with a continuous lawn of oligonucleotides arrayed in millions of 2 x 2 µm barcoded squares without gaps, achieving single cell–scale spatial resolution. The data is output at 2 µm, as well as multiple bin sizes. The 8 x 8 µm bin is the recommended starting point for visualization and analysis.

FAQ

What is the Capture Area architecture for Visium HD slides?

Visium HD Spatial Gene Expression slides contain two 6.5 x 6.5 mm Capture Areas with a continuous lawn of oligonucleotides arrayed in millions of 2 x 2 µm barcoded squares without gaps, achieving single cell–scale spatial resolution.

What samples are compatible with Visium HD?

Visium HD is compatible with human and mouse formalin-fixed paraffin-embedded (FFPE) tissue sections containing mRNA. This includes archived FFPE blocks and pre-sectioned tissue on glass slides. We plan to expand sample access to other tissue conditions in the future.

What tissue stains are compatible with Visium HD?

Visium HD Spatial Gene Expression is compatible with hematoxylin and eosin (H&E) staining for morphological information and with immunofluorescence (IF) staining for protein co-detection.

How can I analyze my Visium HD Spatial Gene expression data?

10x Genomics provides two types of software to help you analyze your data: Space Ranger and Loupe Browser. Space Ranger is an analysis software that automatically overlays spatial gene expression information on your tissue image and identifies clusters of barcoded squares with similar transcription profiles. You can then use Loupe Browser, a visualization software, to interactively explore the results.

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