Comprehensive antigen-specific clonotype discovery in one week
BEAM (Barcode Enabled Antigen Mapping) is a multiplexed antigen screening workflow that empowers rapid discovery of antigen-specific B-cell (BEAM-Ab) and T-cell (BEAM-T) clonotypes. Obtain comprehensive antigen-specific cellular profiles including full-length paired V(D)J sequences, gene expression, and cell surface proteins at single cell resolution to identify rare, antigen-reactive T and B cells for accelerating therapeutic discovery.
Fast track B- and T-cell discovery
BEAM-Ab
Transform antibody discovery with the fastest hit generation workflow, powered by multiplexed antigen screening against thousands of single B cells.
- Go from sample to high-quality antibody hits in under a week
- Screen up to 15 antigens in parallel to generate tens to hundreds of hits per sample
- Easily identify the ideal antibody candidates for downstream validation
BEAM-T
Antigen-specific T cells don’t have to be hard to find anymore. Map antigens to T-cell receptors with off-the-shelf ease and unparalleled cellular characterization.
- Leverage kitted, custom loadable MHC monomers to prepare peptide-MHC complexes in minutes
- Design and source peptides from any vendor for maximum flexibility
- Detect rare and/or therapeutically relevant TCR clonotypes with gene expression at single cell resolution
Workflow
Prepare your sample
Label your cells with barcoded antigen assemblies as recommended in the sample prep user guide. Antigen-specific cell populations can be enriched through flow sorting. Compatible cell suspensions include samples from PBMCs, B cells, T cells, mouse splenocytes, and more.
Construct your 10x library
Construct 10x barcoded libraries using our reagent kits and a compatible Chromium instrument. Each member of the Chromium instrument family encapsulates each cell with a 10x barcoded Gel Bead in a single partition. Within the nanoliter-scale partitions, cells undergo reverse transcription to generate cDNA, which shares a 10x Barcode with all cDNA from its individual cell of origin.
Sequence
The resulting 10x barcoded libraries are compatible with standard NGS short-read sequencing on Illumina sequencers for immune and transcriptional profiling of thousands of individual cells.
Analyze your data
Convert raw sequencing data to biologically meaningful insights with Cell Ranger. Cell Ranger processes data from gene expression, paired immune receptor repertoire sequences, cell surface proteins, and antigen specificities.
Visualize your data
Use our Loupe V(D)J Browser software to define and compare cell types based on different marker types.
