Novogene sRNA-seq service is an effective approach to selectively target any species of sRNAs with unprecedented sensitivity and high resolution, all in a single analysis. Coupled with a robust in-house bioinformatics pipeline, Novogene sRNA-seq service has been assisting researchers to describe the differential expression of miRNAs, structural alterations, and to discover novel sRNAs.
Applications
- sRNA-seq has been supporting many compelling research avenues, including:
- Profiling known and novel transcripts and expression quantifications of small RNA
- Predicting targeting genes of miRNA candidates
- Identifying tissue, stage, or cell type-specific biomarkers for cancer/disease diagnostics and classification
- Discovering regulatory networks and understanding the mechanisms of tissue or organism development based on transcript profiles
Benefits
The Novogene sRNA-seq is a well-established service that relies on extensive experience, with numerous samples successfully sequenced. It allows access to expression profiles and transcript characterizations for multiple small RNA species, with a tried-and-tested workflow for both input sample of total RNA and exosomal RNA.
Novogene offers comprehensive analyses using top-notch software and a mature in-house pipeline, meeting all your bioinformatics needs. Researchers can obtain the regulation network, the deep regulation mechanism of gene expression, and more, with Novogene’s high quality services.
Specifications: RNA Sample Requirements
| Library Type | Sample Type | Amount | RNA Integrity Number (Agilent 2100) | Purity (NanoDrop) |
| Small RNA Library | Total RNA | ≥ 2 μg | Animal ≥ 7.5, Plant ≥ 7, with smooth baseline | A260/280 = 1.8-2.2 A260/230 ≥ 1.8 |
| Exosomal Small RNA Library | Exosomal RNA | ≥ 10 ng | Peak between 25-200 nt, FU> 10, no peak > 2000 nt |
Specifications: Sequencing and Analysis
| Sequencing Platform | Illumina NovaSeq 6000 |
| Read Length | Single-end 50 bp |
| Recommended Sequencing Depth | ≥ 10 million read pair per sample |
| Standard Analysis (miRNA) | Data Quality Control
Summary of Length Distribution Common & Specific Sequence Summary Identification & Characterization of miRNAs Classification & Annotation of miRNA Quantification & Differential Expression Analysis Functional Enrichment Analysis |
Project Workflow
The project workflow starts with sample quality control (Sample QC) to ensure that your samples meet the criteria of the sRNA-Seq technique. Then, the appropriate library is prepared according to your target organism and application and subsequently tested for its quality (Library QC). Next, a single-end 50 bp sequencing strategy is used to sequence the samples and the resulting data is also checked for its quality (Data QC). Finally, bioinformatic analyses are performed and publication-ready results are provided.
