Unify the transcriptome and epigenome in every cell
Simultaneously profile gene expression and open chromatin from the same cell with Chromium Single Cell Multiome ATAC + Gene Expression. Multiply your power of discovery to characterize cell types and states, and uncover gene regulatory programs.
Uncover hidden insights
Multiomic profiling of the transcriptome and epigenome at single cell resolution can transform your understanding of biology. Enhance your characterization of cell types and states, and gain deeper insights into underlying gene regulatory mechanisms with two readouts from every cell. Get started with Single Cell Multiome ATAC + Gene Expression and multiply your power of discovery.
What you can do
- Deeper characterization of cell types and states: Characterize complex cell populations and capture cellular heterogeneity to uncover hidden insights with combined epigenomic and gene expression profiling. Leverage gene expression markers to more easily interpret epigenetic profiles.
- Discover new gene regulatory interactions: Combine discovery of regulatory elements with gene expression to explore gene regulatory interactions driving cell differentiation, development, and disease.
- Make the most of your precious samples: Maximize insights from your limited samples by obtaining transcriptome and open chromatin information from the same cell.
- Explore disease complexity: Understand how gene regulatory networks are disrupted in disease. Interrogate epigenetic remodeling and mechanisms of therapeutic resistance.
Workflow
Prepare your sample
Start with a nuclei suspension isolated from cell culture, primary cells, or fresh or frozen tissue.
Construct your 10x library
Construct a 10x barcoded library using our reagent kits and a compatible Chromium instrument. Each member of the Chromium instrument family encapsulates each cell with a 10x barcoded Gel Bead in a single partition. Within each nanoliter-scale partition, cells undergo reverse transcription to generate cDNA, which shares a 10x Barcode with all cDNA from its individual cell of origin.
Sequence
The resulting 10x barcoded single cell gene expression and ATAC-seq libraries are compatible with standard NGS short-read sequencing on Illumina sequencers, for massively parallel profiling of thousands of individual cells.
Analyze Your Data
Our Cell Ranger ARC analysis software generates open chromatin and gene expression profiles for each cell, identifies clusters of cells with similar profiles, and calculates linkages between open chromatin peaks and gene expression.
Visualize Your Data
Use our Loupe Browser visualization software to interactively explore your results, compare chromatin accessibility and gene expression across cell types and states, and investigate linkages between open chromatin peaks and gene expression.
