How can you ensure that your single cell RNA sequencing (scRNA-seq) studies have the best chance of success? As with so...

Cell heterogeneity is a key contributor to biological complexity that is often masked by bulk techniques, such as RNA se...

Looking back on 2023, we’re amazed and humbled to see the profound impact of Visium Spatial technology across a range of pioneering research endeavours. From uncovering novel T-cell states in cancer to deciphering genes crucial for palate development, each publication exemplifies Visium’s capacity to unveil spatially resolved, whole-transcriptome insights that redefine our understanding of complex biological processes. These studies not only highlight the power of unbiased spatial analysis but also underscore Visium’s role in advancing biomedical research by enabling new discoveries and therapeutic possibilities.

Researchers from the University of Tokyo, University of Ryukyus, and Daiichi Sankyo Co. used single-cell technologies, including Chromium Single Cell ATAC and Gene Expression assays, to uncover the mechanisms of action and resistance of valemetostat, a dual inhibitor targeting EZH1 and EZH2, in treating adult T-cell leukemia/lymphoma (ATL). They found that valemetostat reduces H3K27me3 levels, leading to chromatin relaxation and reactivation of tumor suppressor genes, which improves patient outcomes. Additionally, the treatment increases expression at previously repressed gene loci, countering the epigenetic repression observed in cancer cells. By exploiting Chromium Single Cell ATAC, these findings open a new realm of therapy development, offering promising directions for more effective and durable cancer treatments.

Genuity Science implemented six Hamilton platforms to automate their NGS workflows from DNA quality control to library pooling. This automation offers significant benefits, including increased throughput, enhanced precision, and improved consistency, while reducing manual errors and integrating seamlessly with Laboratory Information Management Systems (LIMS) for effective sample tracking.

Despite being a chronic, multifaceted autoimmune disease that can lead to several comorbidities, psoriasis has no standard treatment guidelines in Vietnam. Besides, it is also commonly mistaken for atopic dermatitis (AD) or clinically ambiguous rashes. This phenomenon raises the need to distinguish these diseases to improve diagnosis and prescribed treatments.By using single-cell technology, researchers have demonstrated that the presence of tissue-resident memory T-cell 1 (Trm1) is highly correlated with AD compared to psoriasis. An open-source web tool called RashX has been introduced, enabling researchers to upload 10x Genomics scRNA-seq immune cell dataset matrices and see how well their sample’s genetic signature aligns with other known AD or psoriasis samples. Although this tool is simple, it serves as a proof-of-concept for how single-cell research can contribute to precision medicine for the diagnosis and treatment of chronic inflammatory skin diseases.

Biomarkers for Prostate Cancer - Beyond the PSA test Biomarkers can be DNA, RNA, proteins, or chemicals produced by t...

The advent of single cell assays, such as 3'GEX and 5'GEX, has revolutionized our research in various fields like oncology, developmental biology, and immunology by enabling detailed gene expression analysis at a single-cell resolution. However, their reliance on fresh samples limited their application. Chromium Fixed RNA profiling- a highly sensitive probe-based technology- overcomes these challenges by allowing sample preservation and transport without the quality losses, simplifying logistics. It enhances efficiency by enabling sample batching and multiplexing. The technology also barcodes up to 1 million cells in 6 minutes, reducing hands-on time and errors. Additionally, its compatibility with FFPE tissues and integration with Visium and Xenium spatial solutions provides comprehensive disease insights, pushing the boundaries of innovation in research and beyond.

Cancer is a multifaceted disease driven by the complex interplay of diverse immune cells. To elucidate the heterogeneity of tumor microenvironments and assess the efficacy of innovative therapeutics, leveraging Chromium Single Cell Multiome ATAC + Gene Expression for comprehensive epigenetic profiling is essential. This advanced technique is pivotal in deciphering the mechanisms of gene activation and silencing that propel cancer progression.

Whole transcriptome spatial gene expression analysis is a transformative method that provides researchers with unparalle...

With a commitment to innovation and customer-centric services, NFI plays a pivotal role in bolstering the rule of law, both domestically and internationally. Through continuous investment in education and innovation, NFI remains at the forefront of forensic research, ensuring rapid adaptation to evolving social, technological, and scientific landscapes within the industry.

BioBank Dresden (BBD), established in 2017, plays a pivotal role in facilitating innovative cancer research by providing high-quality biospecimens to the scientific community.